Sex-specific term change around the areas
good, Smoothed lineplot exhibiting the number of DEGs anywhere between male and female pet at each and every age. Self-confident (negative) opinions depict up-controlled (down-regulated) genes. Grey lines: some other architecture. b, Heatmap icon out of (a). c, mRNA term regarding Apoe in the GAT and you will Axin2 from inside the spleen. Black line: LOESS regression. n=45 (GAT) and n=47 (spleen) independent trials. d, Venn diagrams portraying the brand new overlap out-of DEGs anywhere between ladies and you can men imagined from the 3mo and 18mo of age inside the GAT, SCAT, liver and you may renal. One-sided Fisher’s specific attempt. *** Pe-h, Top Wade terminology enriched among DEGs ranging from ladies and you may males on 18mo old from inside the GAT (e), SCAT (f), liver (g) and you may renal (h). Form ± SEM. n=2 (females) & n=cuatro (males) separate dogs for every single body organ. q-thinking estimated which have Benjamini-Hochberg for each and every databases by themselves, and for Go classes (unit function, cellular part, biological procedure) separately.
For every of the 17 body organs (rows), the typical trajectory of your 15,000 most very expressed genes is actually represented regarding step 1 st line and you can unsupervised hierarchical clustering was utilized so you can classification genetics with similar trajectories (articles dos).